Safieh  Golkhani, Mehran  Majlesi, Mehrdad  Modaresi, 
    The Effect of Balm's Hydro Alcoholic Extract on Activity of Liver Enzymes and Liver Tissue,
    Research Journal of Applied Sciences,
    Volume 8,Issue 9,
    2013,
    Pages 435-438,
    ISSN 1815-932x,
    rjasci.2013.435.438,
    (https://makhillpublications.co/view-article.php?doi=rjasci.2013.435.438)
    Abstract: Balm (<I>Melissa officinalis</I>) is a fragrant plant from 
  Laminacea family which has many medical properties including relaxing, anti-oxidant 
  and anti-bacterial. This study was conducted to investigate the effect of hydro 
  alcoholic extract of balm on activity of liver enzymes including AST, ALT and 
  ALP and also liver tissues variation in Balb/C mice. Samples were randomly divided 
  into five groups (three treatment groups, placebo and control group) with eight 
  members in each group. Groups were kept under similar conditions. Hydro alcholic 
  extract was prepared in three doses (50,100 and 200 mg kg<SUP>-1</SUP>) and 
  were IP for 20 days. Normal saline was used for placebo group. After the last 
  injection blood samples were taken and liver tissues were separated. Changes 
  of AST, ALT and ALP enzymes was evaluated using one way variance analysis and 
  SPSS program (p&lt;0.05). Tissue changes of liver were studied using coloring 
  with H&amp;E technique. According to results, ALT and ALP enzyme was increased 
  (p&lt;0.05) in 200 mg kg<SUP>-1</SUP> dose. Hepatocytes number and histology 
  of liver didn&#146;t show significant variations. On the whole, hydro alcoholic extract 
  of balm could have conservative effects via reducing ALT and ALP enzymes and 
  no harmful effects on liver tissue. So, it can play an important role in preventing 
  and curing diseases in traditional and also current medicine.
    Keywords: mice;liver tissue;liver enzymes;Balm;placebo group