S.H.  Lim, I.  Darah, M.L.  Choo, 
    Influence of Physical and Chemical Parameters on Protease Production by <I>Bacillus megaterium</I> IBRL MS 8.2 in Submerged Fermentation,
    Research Journal of Biological Sciences,
    Volume 8,Issue 2,
    2013,
    Pages 54-61,
    ISSN 1815-8846,
    rjbsci.2013.54.61,
    (https://makhillpublications.co/view-article.php?doi=rjbsci.2013.54.61)
    Abstract: Proteases are a group of enzymes that catalyze the hydrolysis of protein and among the various proteases, bacterial proteases are the most significant when compared to animal and fungal proteases. In this study, a local bacterial isolate <I>Bacillus megaterium</I> IBRL MS 8.2 was found to produce the highest protease production of 155.38 U mL<SUP>-1</SUP> when cultivating it in the improved medium which consisted of initial medium pH of 7.5, inoculum size of 7.5% (3x10<SUP>7</SUP> cells mL<SUP>-1</SUP>), agitation speed of 150 rpm, incubation temperature of 30&deg;C, 0.5% of sucrose and 1.0% of beef extract. The highest protease production was achieved on the 30 h of cultivation. On the other hand, the highest cell growth of 2.33 g L<SUP>-1</SUP> was achieved on 18 h of cultivation time. The data obtained suggest that enzyme production by <I>B. megaterium</I> IBRL MS 8.2 was not growth dependant.
    Keywords: Bacillus megaterium;submerged fermentation;proteases;enzyme production;Malaysia