Xun  Wang, Haifeng  Liu, Ling  Zhao, Han  Dong, Chunyou  Ning, Xuewei  Li, Hengmin  Cui, 
    Effects of the Crude Polysaccharides from <I>Poria cocos</I> on the Proliferation and Differentiation of 3T3-L1 Cells,
    Research Journal of Biological Sciences,
    Volume 6,Issue 11,
    2011,
    Pages 597-601,
    ISSN 1815-8846,
    rjbsci.2011.597.601,
    (https://makhillpublications.co/view-article.php?doi=rjbsci.2011.597.601)
    Abstract: To study the effects of crude polysaccharides from <I>Poria cocos</I> (PCCP) on proliferation and differentiation of 3T3-L1 preadipocyte. 3T3-L1 cells were treated with culture medium contained different concentrations of PCCP (0, 50, 100 and 200 &#956;g mL<SUP>-1</SUP>) for 48 h and subsequently cell proliferation was assayed by MTT Method. The extent of the differentiation was determined by fluescence spectrophotometry and Oil Red O staining on days 8 after treatment with culture medium supplemented with different concentration PCCP (0, 50, 100 and 200 &#956;g mL<SUP>-1</SUP>). As the results, the different concentration PCCP (50, 100 and 200 &#956;g mL<SUP>-1</SUP>) inhibited to some extent proliferation of 3T3-L1 preadipocytes when compared with the control. But the difference is not significant (p&gt;0.05). And PCCP stimulated cell differentiation into adipocyte at lower concentration (50 and 100 &#956;g mL<SUP>-1</SUP>) (p&lt;0.05) while it inhibited cell differentiation into adipocyte at high concentration (200 &#956;g mL<SUP>-1</SUP>) (p&gt;0.05). It can be concluded from the results that the PCCP was involved in regulation of adipogenesis and lipogenesis.
    Keywords: Poria cocos;polysaccharides;3T3-L1;proliferation;differentiation;China