Mohammad  Karimian, Mehran  Miroliaie, Kamran  Ghaedi, Zohreh  Zahraie, 
    Improve the Amplification of <I>LTP </I>(Lipid Transfer Protein) Gene,
    Research Journal of Biological Sciences,
    Volume 5,Issue 12,
    2010,
    Pages 802-804,
    ISSN 1815-8846,
    rjbsci.2010.802.804,
    (https://makhillpublications.co/view-article.php?doi=rjbsci.2010.802.804)
    Abstract: Lipid Transfer Protein encoded by <I>LTP </I>gene in plants. This is a GC rich gene. These base causes that designed primers for <I>LTP</I> gene produce many secondary structures in annealing step of PCR and eventually does not produce a suitable product. Substances such as betaine, Dimethyl Sulfoxide (DMSO) and Ammonium Sulfate (AMS) can to eliminate this obstacle. In this study, the gradient of each material was placed in the PCR reaction and ultimately the optimum concentration for any of this material was obtained. In between, 4.5 &#956;L betaine (5M), 2.25 &#956;L DMSO (10%) and 2.25 &#956;L AMS in the overall volume of 25 &#956;L of PCR mixture were reported optimal concentrations.
    Keywords: Gradient PCR;secondary structures;betaine;dimethyl sulfoxide;ammonium sulfate buffer;Iran