Peyman  Mikaili, Ali Asghar  Hemmati, Ardeshir  Arzi, Neda  Karampour Sistani, 
    The Hydro-Alcoholic Extract of Pomegranate Seed Has Anti-Inflammatory Effects on Formalin-Induced Inflammation in Rat Hind Paw,
    Research Journal of Biological Sciences,
    Volume 5,Issue 8,
    2010,
    Pages 561-564,
    ISSN 1815-8846,
    rjbsci.2010.561.564,
    (https://makhillpublications.co/view-article.php?doi=rjbsci.2010.561.564)
    Abstract: Several <I>in vivo</I> studies have documented that supplementation with pomegranate fruit extract inhibits inflammatory symptoms. In the present study, the anti-inflammatory properties of total hydro-alcoholic extract isolated from pomegranate seed were investigated in formalin-induced rat paw edema. The studied male Wistar albino rats (n = 30) were divided into 5 groups. Inflammation was created by injection of formalin (2.5%) intraplantarily. Then different doses of extract (100, 200, 400 mg kg<SUP>-1</SUP>) were injected in 3 groups, indomethacin (5 mg kg<SUP>-1</SUP>) was injected in 4th group and control group received 5 mL of normal saline intraperitoneally. About 1-3 h after injection of the extract, indomethacin and saline, the volume of paw was measured in all groups by plethysmometer. The results showed hydro-alcoholic extract of pomegranate seed has dose-dependent anti-inflammatory effect and the highest anti-inflammatory effect was observed in the dose of 400 mg kg<SUP>-1</SUP>.
    Keywords: formalin;rat paw edema;anti-inflammatory;Pomegranate seed;indomethacin;Iran