Zhang Yong  , Tang Hao-ru  , Luo Ya  , Jiang Hao  , Hou Yanxia  , 
    Cloning and Sequencing of Strawberry Chitinase Gene CHI2,
    Research Journal of Biological Sciences,
    Volume 4,Issue 5,
    2009,
    Pages 624-628,
    ISSN 1815-8846,
    rjbsci.2009.624.628,
    (https://makhillpublications.co/view-article.php?doi=rjbsci.2009.624.628)
    Abstract: Two genes fragments from cultivated strawberry (Fragaria x ananassa cv. Toyonaka and cv. Akihime) genomic DNA were isolated by PCR amplification. The study showed that these genes were 1575 bp in length and contained a 988 bp Open Reading Frome (ORF) and 2 extron and 1 intron. The homology of nucleotide and deduced amino acid were 99.05 and 98.56%, respectively. Sequecing revealed that the 2 genes encoded a class chitinase. BLAST analysis showed that the homology of deduced amino acid between strawberry and other species’s CHI2 gene were about 50%. The homology tree showed that it obviously distinguished from the others.
    Keywords: Strawberry;chitinase gene;cloning;sequencing;amino acid;CHI2