B.S.D. Pena , B.D. Vazquez , I.A. Cordova , J.C.A. Cordova , J.M.S. Cordova , Determination of Aflatoxins in Commercial Dog Foods by Immunoaffinity Column, Research Journal of Biological Sciences, Volume 3,Issue 7, 2008, Pages 772-774, ISSN 1815-8846, rjbsci.2008.772.774, (https://makhillpublications.co/view-article.php?doi=rjbsci.2008.772.774) Abstract: The occurrence of aflatoxins B1, B2, G1 and G2 in commercial dog food was investigated. Fourteen dog food cereals-based from dog shops were collected. The extracts were cleaned- up through commercially immunoffinity columns followed by fluorescence detection and pH analyzed. Dry and semi-dry adult food showed pH between 6.0-6.2 in average aflatoxins 24 and 17.5 µg kg 1, respectively. The dry and semi-dry puppy food with pH (6.2- 6.8) and levels ranged from 17-29 µg kg 1; three semi-dry adult food with a pH>6. The 42.85% of total foods were above the maximum limit established in Mexico. These results show the occurrence of aflatoxins, indeed the regulatory aspects still need harmonized; also the importance of pH in the performance of immunoaffinity column. Keywords: Pet food;immunoaffinity columns;aflatoxinas;aflatoxicosis;regulation