Asim A. Osman, Imadeldin E. Aradaib, Omer A. Musa,
Detection of Caprine-Specific Nucleic Acid Sequences in Goat Milk Using Polymerase Chain Reaction,
International Journal of Molecular Medicine and Advance Sciences,
Volume 9,Issue 1,
2013,
Pages 5-9,
ISSN 1813-176x,
ijmmas.2013.5.9,
(https://makhillpublications.co/view-article.php?doi=ijmmas.2013.5.9)
Abstract: This study was carried out to evaluate, PCR-based method for
detection of DNA in goat milk. It utilized primers targeting the mitochondrial
cytochrome b (mtcyt-b) gene which was used as a target DNA for PCR amplification.
For the specific identification of goat mtcyt-b gene, pair of primers
(GSL1, GSR2) were used which produced a 428 base pair (bp) PCR product from
milk samples as well as from peripheral blood. Amplification products were visualized
on ethidium bromide-stained agarose gels. Amplification products were not detected
when the PCR was applied to DNA from animal species including cattle, sheep,
swine, camel, deer, horse, donkey and human which indicates that the 2 pairs
of primers are specific for goat. In conclusion, DNA can be extracted from goat
milk and would be advantageous in the variety of application such as species
identification in milk and milk.
Keywords: rapid detection;DNA;Goat milk;PCR assay;cattle