Moushumi Ghosh  , R. Son  , A. M. Sahilah  , K. Sushil  , W. L. Ooi  , I. Salmah  , S. H. Lim  , Sunita Bansal  , Abhijit Ganguli  , 
    PCR Primers Designed from the UidA Gene Sequence for the Detection of Escherichia coli O157:H7,
    Journal of Food Technology,
    Volume 2,Issue 1,
    2004,
    Pages 59-62,
    ISSN 1684-8462,
    jftech.2004.59.62,
    (https://makhillpublications.co/view-article.php?doi=jftech.2004.59.62)
    Abstract: One of the uniqueness of Escherichia coli O157:H7 is it inability to ferment sorbitol in 24 h and its negative test with the MUG assay, although this organism carried the UidA gene which encodes for -glucuronidase in its chromosome. Primers were designed based on the sequence of the -glucuronidase gene and were evaluated in a polymerase chain reaction assays as a marker to detect E. coli O157:H7. Of the three pairs of primers tested, which produces the estimated product size of 352, 271 and 353 bp respectively, one primer pairs (UidA2F & UidA2R) was found to be more specific and useful as a marker in combination with a published primer for the H7 gene for the detection of Escherichia coli O157:H7.
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