QU  Lei, Jing  Xiaoqi, QU  Cheng, XU  Zhengyu, Song  Xiaoyue, SHI  Lei, LIU  Jiaorong, Dong  Shuwei, LIU  Jinwang, ZHU  Haijing, 
    Correlation Analysis Between Polymorphism of <i>CMTM2</i> Promoter and Growth Measurements
of Shaanbei White Cashmere Goats,
    Journal of Animal and Veterinary Advances,
    Volume 20,Issue 5,
    2021,
    Pages 134-141,
    ISSN 1680-5593,
    javaa.2021.134.141,
    (https://makhillpublications.co/view-article.php?doi=javaa.2021.134.141)
    Abstract: To determine the correlation between the 14-bp
insertion/deletion (InDel) polymorphism in the promoter
of CMTM2 gene and the growth traits of Shaanbei white
cashmere goats. Shaanbei white cashmere nannies
population (n = 1105 including 621 bred goats and 484
adult goats) were chosen. The body weight and body
measurements of the goats were measured and ear tissues
were collected. After the genomic DNA were extracted,
PCR amplification were conducted, agarose gel
electrophoresis and DNA sequencing were carried out to
detect the InDel mutation in the promoter of the <i>CMTM2</i>
gene. The correlation between the InDel characteristics
and growth traits of cashmere goats was analyzed using
SPSS 23.0 Software. In Shaanbei white cashmere goats&#146;
population, three genotypes of II, ID and DD were
detected at the mutation site of <i>CMTM2</i> promoter. The
frequency of allele &#147;I&#148; (0.863) is higher than that of allele
&#147;D&#148; (0.137), this InDel mutation conformed to Hardy
Weinberg equilibrium (p>0.05) and showed low
polymorphism (PIC<0.25). The 14-bp InDel site in the
promoter region was extremely significantly
correlated with the body height, hip width and height
at hip cross in adult goats (p<0.01); further
association analysis results showed that this site was
significantly related with body height and the height
at hip cross (p<0.05) and extremely significantly related
to the hip width (p<0.01) in Shaanbei white cashmere
goat population. The 14-bp InDel in the promoter of
<i>CMTM2</i> gene is significantly related to the body
height, hip width and height at hip cross of Shaanbei
white cashmere goats. This site can be used for Marker
Assisted Selection (MAS) in Shaanbei white cashmere
goats. Also, it provided a theoretical and practical basis
for the breeding of Shaanbei white cashmere goats.
    Keywords: CMTM2 gene;cashmere goat;InDel;growth traits;PCR