M. Lutfi Selcuk, Sadullah Bahar,
The Morphometric Properties of the Lumbar Spinal Cord Segments in Horses,
Journal of Animal and Veterinary Advances,
Volume 13,Issue 11,
2014,
Pages 653-659,
ISSN 1680-5593,
javaa.2014.653.659,
(https://makhillpublications.co/view-article.php?doi=javaa.2014.653.659)
Abstract: The aim of this study was to determine morphometric features
of lumbar segments of the spinal cord of horses. Spinal cords of six horses
from different breeds and sexes were used. Lumbar parts of the cords were dissected
out from vertebral columns and segmentation was performed. Macroscopic measurements
were done on the segments and then routine histological procedure was applied.
Segments were sliced transversally at 3.8 mm thickness. Samples were cut at
10 μm thickness and stained with May-Grunwald-Giemsa. Slides images were
used by stereo investigator. Diameter measurements were carried out and area
and volume calculations were performed by using Cavalieri principle. The weight,
length and volume ratios of the lumbar parts to spinal cord were 14.7, 13 and
14%, respectively. A decrease in lengths of the segments from L1
to L6 was observed. In the microscopic analysis, L4 and
L5 had the biggest transverse diameters however L6 had
the largest vertical diameter. L5 had the largest values of cross-sectional
and white matter area however L6 had the largest value of gray matter.
Volumes of all segments except for L6 were similar to each other
and the largest volume of gray matter and the largest volume of white matter
were observed in L5 and L3, respectively. It was determined
that the volume ratio of gray matter to the volumes of segments increased from
L1 to L6 whereas volume of white matter was decreased
in those segments. In conclusion, the data obtained will contribute to the morphology
of related region and the further studies.
Keywords: lumbar segments;spinal cord;Cavalieri principle;segmental morphometry;gray matter