Ayhan  Filazi, Filiz  Sen, 
    Detection of Penicillin Residues in Cow Milk Using High Performance Liquid Chromatography with UV-Diode Array Detection,
    Journal of Animal and Veterinary Advances,
    Volume 13,Issue 7,
    2014,
    Pages 477-483,
    ISSN 1680-5593,
    javaa.2014.477.483,
    (https://makhillpublications.co/view-article.php?doi=javaa.2014.477.483)
    Abstract: Researchers describe the development of a Novel Method that 
  is sensitive and reliable for detecting isoxazolyl penicillins (Cloxacillin 
  (CLO) and Dicloxacillin (DCL)) in commercially available cow milk using High-Performance 
  Liquid Chromatography with UV-Diode Array Detection (HPLC-DAD). CLO and DCL 
  were extracted via deproteinization of milk samples, adjusting the pH to 4.5-5 
  using hydrochloric acid and then adding acetone/chloroform, followed by centrifugation 
  and cleanup using a 0.45 μm nylon filter. CLO and DCL were separated using 
  an Inertsil ODS-C<SUB>18</SUB> column (4.6x250 mmx3.5 &#956;m) via elution with 
  a mobile phase consisting of 0.05 M phosphate buffer solution at pH 4.7 and 
  acetonitrile at 22&deg;C. Recovery of CLO and DCL was 96.47-106.94 and 97.76-104.86%, 
  respectively. The limit of quantifications were 23.15 &#956;g kg<SUP>-1</SUP> 
  for CLO and 2.28 &#956;g kg<SUP>-1</SUP> for DCL which are lower than the limits 
  for residues in cow milk established by the European Union, indicating that 
  the method is suitable for performing routine analysis. In order to determine 
  this Novel Method&#146;s utility in a laboratory environment for analyzing real 
  raw milk samples 100 raw milk samples were obtained from raw milk tanks belonging 
  to milk producers. CLO and DCL were not detected in any of the samples. The 
  present findings indicate that the Novel Detection Method is simple, rapid, 
  sensitive and reliable, as compared to other detection methods and that as it 
  uses fewer chemicals and does not require derivatization it could be considered 
  the preferred method.
    Keywords: Cloxacillin;dicloxacillin;HPLC;milk;residue