Jinnian Li, Ying Huang, Lu Yao, Lin Li, Xueqin Liu, Anning Huang, Tingting Zhang, Wenping Wang,
Characterization of Antimicrobial Resistance and Enterotoxin Genes in Methicillin-Resistant
Staphylococcus aureus Isolated from Mastitis Milk and Food Poisoning
Cases,
Journal of Animal and Veterinary Advances,
Volume 13,Issue 7,
2014,
Pages 423-429,
ISSN 1680-5593,
javaa.2014.423.429,
(https://makhillpublications.co/view-article.php?doi=javaa.2014.423.429)
Abstract: Enterotoxigenic Staphylococcus aureus is a main cause
of Staphylococcal Food Poisoning (SFP). Here, researchers characterized the
antimicrobial resistance, distribution and expression of enterotoxin genes of
41 Methicillin-Resistant S. aureus (MRSA) isolated from bovine mastitis
milk and SFP cases. Apart from three SFP-acquired isolates which could produce
extended-spectrum β-lactamases, all the others carried mecA gene
and expressed the penicillin-binding protein 2a. The 92.7% of MRSA isolates
tested showed twenty two multi-drug resistant patterns among which SFP-acquired
isolates had higher resistant rate and the MIC50/90 values of cefotaxime,
amikacin, azithromycin, ciprofloxacin and gentamicin than those of milk-acquired
isolates. The 95.1% of MRSA isolates carried at least one Staphylococcal Enterotoxins
(SEs) gene and could produce the corresponding classical SEs, the differences
in the prevalence of enterotoxigenic MRSA observed between milk-acquired and
SFP-acquired isolates were not statistically significant. Six SEs genotypes
were found, among which the genotypes sea-seg-sei-seln-selm and sea-seb-sec-seg-sei-seln-selm
predominated, respectively in milk-acquired and SFP-acquired isolates.
Keywords: enterotoxin gene;antimicrobial resistance;S. aureus;Methicillin-resistant;raw milk;food poisoning