Wang  Binwu, Gao  Yulong, Song  Zhongbang, Li  Wenzheng, 
    Molecular Characterization and Expression Pattern of a Novel Cadmium/Zinc-Transporting <I>ATPase HMA1</I> Gene,
    Journal of Animal and Veterinary Advances,
    Volume 13,Issue 3,
    2014,
    Pages 159-164,
    ISSN 1680-5593,
    javaa.2014.159.164,
    (https://makhillpublications.co/view-article.php?doi=javaa.2014.159.164)
    Abstract: Cadmium (Cd) of tobacco is a pollutant that is extremely toxic 
  to the health of humans. Cadmium/zinc-transporting <I>ATPase HMA1</I> gene has 
  been characterized to function in the plant cadmium/zinc-transporting. The complete 
  coding sequence of tobacco cadmium/zinc-transporting <I>ATPase HMA1</I> gene 
  was amplified by RT-PCR. The open reading frame of tobacco cadmium/zinc-transporting 
  <I>ATPase HMA1</I> gene was 2418 bp which encodes a protein of 805 amino acids. 
  BLAST analysis revealed that tobacco cadmium/zinc-transporting ATPase HMA1 protein 
  shares high homology with the cadmium/zinc-transporting ATPase HMA1 of potato 
  (85%), <I>Lycopersicon esculentum</I> (84%), wine grape (71%), sweet orange 
  (71%), soybean (69%) and thale cress (68%). Results also showed that tobacco 
  cadmium/zinc-transporting <I>ATPase HMA1</I> gene has a closer genetic relationship 
  with the cadmium/zinc-transporting <I>ATPase HMA1</I> gene of <I>Lycopersicon 
  esculentum</I>. Prediction of transmembrane helices showed that tobacco cadmium/zinc-transporting 
  ATPase HMA1 might be a transmembrane protein. The expression profile was studied 
  and results indicated that tobacco cadmium/zinc-transporting <I>ATPase HMA1</I> 
  gene was moderately expressed in root, leaf and stem but hardly expressed in 
  flower. These results established the primary foundation of utilizing tobacco 
  cadmium/zinc-transporting <I>ATPase HMA1</I> gene to descrease the cadmium content 
  of tobacco and benefit the health of humans in the future.
    Keywords: Tobacco;gene;cadmium/zinc-transporting ATPase HMA1;expression pattern;established