Dongying Yang,
A Novel PCR-RFLP (Xba I) Polymorphism Analysis of the Bovine Calpastatin (CAST) Gene in Chinese Luxi Cattle,
Journal of Animal and Veterinary Advances,
Volume 12,Issue 6,
2013,
Pages 788-790,
ISSN 1680-5593,
javaa.2013.788.790,
(https://makhillpublications.co/view-article.php?doi=javaa.2013.788.790)
Abstract: Calpastatin (CAST) is a specific inhibitor of the ubiquitous
calcium-dependent proteases-μ-calpain and m-calpain, found in mammalian
tissues. In the coding region of the bovine CAST gene (CAST) the new
nucleotide sequence polymorphism was found being a substitution G→C at position
962 nt (consensus sequence-GenBank AF281256). Consensus of bovine CAST sequence
with that of human revealed that G→C substitution was located at position 1460
nt of exon 12. Computer analysis of the mutation showed the Ser→Thr substitution
for CAST protein. The frequency of alleles C and G was 0.41 and 0.59, respectively.
The message from these results is that CAST gene may probably be considered
as a candidate marker for beef quality.
Keywords: Genetic polymorphisms;CAST gene;haplotype;RFLP;cattle