Xueliang  Zhao, Yugang  Gao, Pu  Zang, He  Yang, Tong  Liu, Qun  Liu, Lianxue  Zhang, 
    Screening of Ginseng Proteins Interacted with BVDV E0 Protein,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 6,
    2013,
    Pages 699-704,
    ISSN 1680-5593,
    javaa.2013.699.704,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.699.704)
    Abstract: Bovine Viral Diarrhea Virus (BVDV), a single-stranded RNA 
  virus can cause fatal diarrhoea syndrome, respiratory problems and reproductive 
  disorder in herd. However, there still no effective vaccine or medicines for 
  controlling the spreading of BVDV. In this study, researchers are interested 
  in obtaining proteins with anti-BVDV activity in ginseng. The structure protein 
  E0 of BVDV was used as bait protein to screen the ginseng cDNA library by the 
  yeast two hybrid assay system. The result showed that eukaryotic translation 
  initiation factor 5A (eIF5A) of ginseng has direct interaction with E0. The 
  interaction was verified by GST-pull down analysis. This interaction provides 
  a target for researching of antiviral drugs for controlling the spreading of 
  BVDV.
    Keywords: Bovine viral diarrhea virus;ginseng;yeast two hybrid;cDNA library;reproductive disorder