Yilmaz  Ciftci, Oguzhan  Eroglu, Sirin  Firidin, 
    Determination of Sturgeon Species Living in the Black Sea Coasts of Turkey 
  by
Sequence, RFLP and Multiplex PCR Analysis Methods,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 6,
    2013,
    Pages 676-682,
    ISSN 1680-5593,
    javaa.2013.676.682,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.676.682)
    Abstract: In order to perform checks in the trade of fish species under 
  the threat of extinction and with commercial importance such as sturgeon, the 
  fish must be identified rapidly and precisely from any tissue and for this purpose 
  morphometric and protein based determinations can be inadequate. In this study, 
  <I>Acipenser stellatus</I>, <I>Acipenser gueldenstaedtii</I> and <I>Huso huso</I> 
  sampled from Trabzon coasts were used. For RFLP and sequence analysis, the mitochondrial 
  Cytochrome-b (<I>mtDNA Cyt-b</I>) gene was amplified by the help of PCR. From 
  Cyt-b sequence data, it was observed that in the Maximum Parsimony (MP) dendrogram, 
  99% of the species were separated. Furthermore in the Cyt-b PCR product restriction 
  enzyme analyses, it was determined that Hinf I and Rsa I enzymes were distinguishing 
  for 3 species. In the multiplex PCR application in the mtDNA D-loop region, 
  the product lengths were determined as 420 bp for <I>A. gueldenstaedtii</I>, 
  350 bp for <I>H. huso</I> and 260 bp for <I>A. stellatus</I> and they are distinctive 
  for the species. The results showed that the multiplex PCR application is a 
  method which is cheap and effective for the identification of sturgeon species 
  in short duration.
    Keywords: Sturgeon;mitochondrial DNA;multiplex PCR;PCR-RFLP;sequence;species identification