Woo-Shin  Lee, Naw May Lay  Thant, Baek-Jun Kim  , Hyeon-Seo Ko  , Khin Mar Yi  , 
    Molecular Phylogenetic Analysis of Non-Invasive Samples for the Endangered Phayre’s Leaf Monkey 
  (<I>Trachypithecus phayrei</I>) in Popa Mountain Park, Central Myanmar,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 5,
    2013,
    Pages 625-632,
    ISSN 1680-5593,
    javaa.2013.625.632,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.625.632)
    Abstract: Phayre&#146;s leaf 
  monkey (<I>Trachypithecus phayrei</I>) is an endangered endemic species distributed 
  predominantly in Southeast Asia. Although, three subspecies (<I>T. p. phayrei</I>, 
  <I>T. p. crepusculus</I> and <I>T. p. shanicus</I>) are known, molecular phylogenetic 
  studies on this monkey are still limited. In Myanmar, there was a controversy 
  for the species identity of Phayre&#146;s 
  leaf monkey inhabiting the Popa Mountain Park (PMP). Here, 32 non-invasive fecal 
  samples and one non-destructive bone sample were used to infer the phylogenetic 
  status of <I>T. phayrei</I> from PMP. Two DNA markers, nuclear Protamine P1 
  (Prm1) and mitochondrial Cytochrome b (Cyt-b) were selected for PCR and sequencing. 
  Three haplotypes for <I>Cyt-b</I> gene and two unique sequences for <I>Prm1</I> 
  gene were detected from 33 samples. The <I>Cyt-b</I> phylogenetic trees showed 
  that the population of Phayre&#146;s 
  leaf monkey in PMP is more closely related to the subspecies <I>T. p. shanicus</I>. 
  However, the Prm1 phylogenetic trees could not resolve the phylogenetic position 
  of <I>T. phayrei</I> subspecies. The results suggest the population from PMP 
  as the subspecies <I>T. p. shanicus</I> but further taxonomic studies for all 
  populations of this threatened monkey in Myanmar should be recommended for the 
  species&#146; conservation and management.
    Keywords: Cytochrome b;non-invasive sample;phylogeny;protamine P1;Trachypithecus phayrei;Myanmar