Ruofeng  Yan, Zhikai  Zhang, Lixin Xu  , Xiaokai Song  , Xiangrui Li  , 
    Characterization of HcMSP, A Novel Gender Specific Gene from Parasitic Nematode<I> Haemonchus contortus</I>,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 5,
    2013,
    Pages 593-599,
    ISSN 1680-5593,
    javaa.2013.593.599,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.593.599)
    Abstract: Major Sperm Protein (MSP) is one of the important components 
  of nematode sperms and the characters of this protein were widely studied in 
  <I>Caenorhabdit elegans</I> and <I>Ascaris suum</I>. However, no knowledge of 
  homology proteins in <I>Haemonchus contortus</I> was reported. In the present 
  research, the <I>MSP </I>gene of this parasite (HcMSP) was first cloned and 
  characterized. A pair of primer targeted the conserved sequence of nematode 
  MSPs was designed and a partial of <I>HcMSP</I> gene was obtained by reverse 
  transcription PCR (RT-PCR). Then, the 5' and 3' ends of <I>HcMSP</I> gene were 
  cloned by 5' Rapid Amplification of cDNA ends (RACE) and 3' RACE. The full length 
  of cDNA was obtained by overlapping the sequences of 3' and 5' extremities. 
  The Open Reading Frame (ORF) was amplified by RT-PCR and expressed in prokaryotic 
  cell.
    Keywords: Haemonchus contortus;major sperm protein;RACE;differential expressions;gene