Zhao  Leifeng, Nian  Fuzhao, 
    Molecular Characterization and Expression Pattern of a Novel Flavonoid 3'-Hydroxylase 
  Gene,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 16,
    2013,
    Pages 1352-1356,
    ISSN 1680-5593,
    javaa.2013.1352.1356,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.1352.1356)
    Abstract: Flavonoids have significant health benefits for animals and humans. Flavonoid 3'-hydroxylase gene have been characterized to be required for flavonoid biosynthesis. In present experiment, the complete mRNA sequence of tobacco flavonoid 3'-hydroxylase gene was amplified using the rapid amplification of cDNA ends methods. The full-length tobacco flavonoid 3'-hydroxylase gene mRNA was 1828 bp containing an 1578 bp open reading frame which encodes a protein of 515 amino acids. Sequence analysis revealed that the flavonoid 3'-hydroxylase genes are conserved in plants. Results also showed that tobacco flavonoid 3'-hydroxylase gene has a closer genetic relationship with the flavonoid 3'-hydroxylase gene of <I>Lycopersicon esculentum</I>. Prediction of transmembrane helices showed that tobacco flavonoid 3'-hydroxylase might be a transmembrane protein. The expression profile was studied and the results indicated that tobacco flavonoid 3'-hydroxylase gene was highly expressed in in leaf and flower. These results established the primary foundation of utilization of tobacco flavonoids in the future.
    Keywords: Flavonoid;tobacco;flavonoid 3`-hydroxylase;expression pattern;flower