Jun  Li, Jian-Li  Shi, Xiao-Yan  Cong, Xiao-Yuan  Yuan, Jia-Qiang  Wu, Wen-Bo  Sun, Yi-Jun  Du, Zhe  Peng, Jin-Bao  Wang, Bao-Hua  Huang, Dai-Cheng  Liu, 
    Methods Compare for Rescued Porcine Circovirus Type 2 Viruses,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 15,
    2013,
    Pages 1296-1298,
    ISSN 1680-5593,
    javaa.2013.1296.1298,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.1296.1298)
    Abstract: Porcine Circovirus type 2 (PCV2), the essential causative 
  agent of the Post weaning Multisystemic Wasting Syndrome (PMWS) has posed a 
  grave threat to global swine industry in recent decades. Infectious molecular 
  clone is the best way to get the purificated virus for pathogenesis study and 
  inactivated vaccine produce. The purpose of this study was to compare and get 
  the best way for PCV2 infectious molecular clone to rescue viruses. In this 
  study, four PCV2 infectious molecular clone was constructed and transfected 
  into PK-15 cells. IFA and Real-time PCR was used to detect the presence of PCV2. 
  The IFA results revealed the precence of rescued viruses. The real-time PCR 
  results showed that there were the most DNA copies with Ligation DNA transfection, 
  the least DNA copies with pEASY-PCV2 transfection and almost the same with psk-2PCV2 
  and psk-PCV2 transfection. This assay afford the best way to construct and get 
  PCV2 infectious molecular clone for further research.
    Keywords: Rescue;porcine circovirus type 2;infectious molecular clone;real-time PCR;cell