Zhao  Leifeng, Nian  Fuzhao, Zhang  Yonghui, Luo  Dingqi, Xia  Jianhua, Jiang  Sheng, 
    Molecular Characterization and Expression Pattern of a Novel <I>NMNAT1</I> 
  Gene,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 15,
    2013,
    Pages 1281-1285,
    ISSN 1680-5593,
    javaa.2013.1281.1285,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.1281.1285)
    Abstract: Nicotinamide adenine dinucleotide is important to the health 
  of animals and humans. Nicotinamide Mononucleotide Adenylyltransferase 1 (<I>NMNAT1</I>) 
  gene has been characterized to be required for nicotinamide adenine dinucleotide 
  biosynthesis. In present experiment, the complete mRNA sequence of tobacco <I>NMNAT1</I> 
  gene was amplified using the rapid amplification of cDNA ends methods. The full-length 
  tobacco <I>NMNAT1</I> gene mRNA was 1,445 bp containing a 753 bp open reading 
  frame which encodes a protein of 250 amino acids. BLAST analysis revealed that 
  tobacco NMNAT1 protein shares high homology with the NMNAT1 of <I>Lycopersicon 
  esculentum</I> (87%), wine grape (65%), soybean (65%), cacao (65%) and barrel 
  medic (62%). Results also showed that tobacco <I>NMNAT1</I> gene has a closer 
  genetic relationship with the <I>NMNAT1</I> gene of <I>lycopersicon esculentum</I>. 
  The expression profile was studied and the results indicated that tobacco <I>NMNAT1</I> 
  gene was highly expressed in leaf. These results established the primary foundation 
  of utilizing tobacco nicotinamide adenine dinucleotide or NMNAT1 as drugs for 
  animals and humans in the future.
    Keywords: Tobacco;gene;NMNAT1;expression pattern;dinucleotide