Zhao  Leifeng, Jiang  Sheng, Xia  Jianhua, Luo  Dingqi, Zhang  Yonghui, Nian  Fuzhao, 
    Molecular Characterization and Expression Pattern of a Novel Peroxisomal Nicotinamide Adenine Dinucleotide Carrier Gene,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 15,
    2013,
    Pages 1276-1280,
    ISSN 1680-5593,
    javaa.2013.1276.1280,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.1276.1280)
    Abstract: Nicotinamide adenine dinucleotide have significant health 
  benefits for animals and humans. Peroxisomal nicotinamide adenine dinucleotide 
  carrier gene functions in mediating the nicotinamide adenine dinucleotide import 
  into peroxisomes. The complete coding sequence of tobacco peroxisomal nicotinamide 
  adenine dinucleotide carrier gene was amplified by RT-PCR. The open reading 
  frame of tobacco peroxisomal nicotinamide adenine dinucleotide carrier gene 
  was 1017 bp which encodes a protein of 338 amino acids. Sequence analysis revealed 
  that the peroxisomal nicotinamide adenine dinucleotide carrier of tobacco shares 
  high homology with the peroxisomal nicotinamide adenine dinucleotide carrier 
  of <I>Lycopersicon esculentum</I> (88%), <I>Fragaria vesca</I> subsp. vesca 
  (74%), thale cress (73%), chickpea (73%) and foxtail millet (67%). Phylogenetic 
  tree analysis revealed that the tobacco peroxisomal nicotinamide adenine dinucleotide 
  carrier gene has a closer genetic relationship with that of <I>Lycopersicon 
  esculentum</I>. Prediction of transmembrane helices showed that tobacco peroxisomal 
  nicotinamide adenine dinucleotide carrier might be a transmembrane protein. 
  Expression profile was studied and the results indicated that tobacco peroxisomal 
  nicotinamide adenine dinucleotide carrier gene was highly expressed in stem, 
  leaf and flower. These results established the primary foundation of using tobacco 
  nicotinamide adenine dinucleotide as drug for animals and humans in the future.
    Keywords: expression pattern;peroxisomal nicotinamide adenine dinucleotide carrier;gene;Tobacco;millet