Zhao  Leifeng, Nian  Fuzhao, Zhang  Yonghui, Luo  Dingqi, Xia  Jianhua, Jiang  Sheng, 
    Molecular Characterization and Expression Pattern of a Novel Nicotinamide Adenine Dinucleotide Transporter Gene,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 15,
    2013,
    Pages 1271-1275,
    ISSN 1680-5593,
    javaa.2013.1271.1275,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.1271.1275)
    Abstract: Nicotinamide adenine dinucleotide is a drug which is important 
  to the health of animals and humans. Nicotinamide adenine dinucleotide transporter 
  1 gene have been characterized to be required for nicotinamide adenine dinucleotide 
  bioactivety. In present experiment, the complete mRNA sequence of tobacco nicotinamide 
  adenine dinucleotide transporter 1 gene was amplified using the rapid amplification 
  of cDNA Ends Methods. The full-length tobacco nicotinamide adenine dinucleotide 
  transporter 1 gene <I>mRNA</I> was 1,588 bp containing an 948 bp open reading 
  frame which encodes a protein of 315 amino acids. BLAST analysis revealed that 
  tobacco nicotinamide adenine dinucleotide transporter 1 protein shares high 
  homology with the nicotinamide adenine dinucleotide transporter 1 of <I>Lycopersicon 
  esculentum</I> (93%), wine grape (86%), soybean (81%), chickpea (79%) and foxtail 
  millet (78%). Results also showed that tobacco nicotinamide adenine dinucleotide 
  transporter 1 gene has a closer genetic relationship with the nicotinamide adenine 
  dinucleotide transporter 1 gene of <I>Lycopersicon esculentum</I>. Prediction 
  of transmembrane helices showed that tobacco nicotinamide adenine dinucleotide 
  transporter 1 might be a transmembrane protein. The expression profile was studied 
  and the results indicated that tobacco nicotinamide adenine dinucleotide transporter 
  1 gene was highly expressed in leaf. These results established the primary foundation 
  of using tobacco nicotinamide adenine dinucleotide as drug for animals and humans 
  in the future.
    Keywords: Tobacco;nicotinamide adenine dinucleotide transporter 1;expression pattern;protein;wine