Hai  Fang, Qiumei  Shi, Yanying  Zhang, QiuYue  Wang, Guisheng  Gao, Guangping  Gao, Donglin  Zhang, Cuizhen  Chen, Xiaomei  Lv, 
    Serotype Distribution and Detection of Enterotoxin Gene of Chicken Source of Salmonella in the Eastern Part of Hebei Province,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 13,
    2013,
    Pages 1183-1189,
    ISSN 1680-5593,
    javaa.2013.1183.1189,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.1183.1189)
    Abstract: To explore the pathogenic mechanism of Salmonella, serotype 
  was identified and enterotoxin gene <I>stn</I> was detected for 47 strains suspected 
  Salmonella in the Eastern part of Hebei Province. According to the bacterial 
  culture characteristic, physicochemical properties and analysis results of K-3401 
  semi automatic bacteria identification instrument and identification for another 
  time by Chengdu Institute of Biological Products, enterotoxin gene <I>stn</I> 
  was detected by PCR. The 17 strains of <I>Salmonella gallinarum</I>, 14 strains 
  of <I>Salmonella typhimurium</I>, 4 strains of <I>Salmonella pullorum</I> 2 
  strains of <I>Salmonella paratyph</I>i A, 2 strains of Salmonella group BO, 
  5 strains of <I>Salmonella bovismorbificans</I>, 3 strains of <I>Salmonella 
  enteritidis</I> were detected from 47 strains of chicken source of Salmonella. 
  The 45 strains of <I>stn</I> gene were amplified successfully in all 47 strains. 
  The carrying rate was 95.7%. Homology of <I>stn</I> gene of test strains of 
  Salmonella was between 94 and 100%. Evolutionary tree display that different 
  serotypes of Salmonella enterotoxin <I>stn</I> gene divided into 5 groups. There 
  was no homology with other bacterium. Chicken source of Salmonella contained 
  7 kinds of serotypes in the eastern part of Hebei Province. Among them, <I>Salmonella 
  gallinarum</I> was 36.2% (17/47), <I>Salmonella typhimurium</I> which was advantages 
  serotypes was 29.8% (14/47). The carrying rate of enterotoxin gene <I>stn</I> 
  which had relation with bacterial pathogenicity was 95.7% (45/47).
    Keywords: Salmonella in the Eastern part of Hebei Province;serotype distribution;enterotoxin stn;polymerase chain reaction;physicochemical