Deshun  Shi, Zhengsan  Wu, Qingyou  Liu, Peng  Zhu, Shuai  Liu, Guihua  Bian, Sufang  Yang, Fenghua  Lu, 
    Effects of Scriptaid on the Histone Acetylation and <I>in vitro</I> Development of Porcine Somatic Cell Nuclear Transfer Embryos,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 13,
    2013,
    Pages 1169-1176,
    ISSN 1680-5593,
    javaa.2013.1169.1176,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.1169.1176)
    Abstract: Effects of Scriptaid (SCR), low toxicity Histone Deacetylase 
  inhibitors (HDACi) on the histone acetylation and development of porcine Somatic 
  Cell Nuclear Transfer (SCNT) embryos were examined in this study. Treatment 
  of activated reconstructed SCNT embryos with 500 nM SCR for 12 h resulted in 
  a significantly increase in the blastocyst development compared to untreated 
  group (21.0 vs 9.7%, p&lt;0.05). Further, SCR treatment resulted in SCNT embryos 
  at the 2, 4-cells and blastocyst stage with higher acetylation level of H3K18 
  in comparison with untreated group and similar to fertilized counterparts. Measurement 
  of four selected developmentally important genes (<I>HDAC2</I>, <I>HAT1</I>, 
  <I>OCT4</I> and Phosphoglycerate Kinase 1 (<I>PGK1</I>)) in blastocysts showed 
  that treatment of SCNT embryos with SCR resulted in an increase expression in 
  HAT1 and decrease expression in HDAC2 and PGK1. A similar expression profile 
  of these genes was found in blastocysts developed from SCR treated SCNT embryos 
  and <I>In Vitro </I>Fertilization (IVF) embryos. These results suggest that 
  SCR treatment can improve the development competence of porcine SCNT embryos 
  and result in a similar expression pattern of genes related to histone acetylation 
  and acetylation level of histone compared to IVF embryos.
    Keywords: Development;histone acetylation;porcine;scriptaid;somatic cell nuclear transfer