J.G.  Chen, A.X.  Liang, L.  Han, J.J.  Xiong, A.Z.  Guo, L.G.  Yang, 
    The Effect of <i>Helicobacter pylori</i> on Apoptosis of the GES-1 Transfected pEGFP-N1-UreB Eukaryotic Expression Plasmid <i>in vitro</i>,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 23,
    2012,
    Pages 4435-4440,
    ISSN 1680-5593,
    javaa.2012.4435.4440,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.4435.4440)
    Abstract: To investigate the effect of <I> Helicobacter pylori</I> on 
  apoptosis of the GES-1 transfected pEGFP-N1-UreB eukaryotic expression plasmid 
  <I>in vitro</I>. The <I>Hp-UreB</I> gene was amplified by PCR and cloned into 
  eukaryotic expression plasmid pEGFP-NI to construct recombinant plasmid pEGFP-NI-UreB 
  and transfected into GES-1 cells and screened by G418. GES-1 cells transfected 
  into plasmid pEGFP-NI-UreB were co-cultured with <I>H. pylori</I> SS1 and its 
  apoptosis was quantified by flow cytometry using Annexin V-APC and 7-AAD. Changes 
  in cell morphology were observed by transmission electron microscope. The recombinant 
  pEGFP-N1-UreB was constructed and stable transfected into GES-1 cells. The apoptotic 
  rates of cells transfected into pEGFP-N1-UreB plasmids were significantly lower 
  than those of control cells (p&lt;0.05).
    Keywords: pEGFP-N1-UreB;H. pylori SS1;GES-1;apoptosis;China