Feng Wang, Weiwei Wu, Hanikezi , Kechuan Tian, Xinming Xu, Yuezhen Tian,
The Evaluation of Factors Affected on Juvenile in vitro Embryo Transfer in Xinjiang Fine Wool Lamb,
Journal of Animal and Veterinary Advances,
Volume 11,Issue 22,
2012,
Pages 4143-4146,
ISSN 1680-5593,
javaa.2012.4143.4146,
(https://makhillpublications.co/view-article.php?doi=javaa.2012.4143.4146)
Abstract: This research was conducted to determine the effect of FSH
made in Canada and Ningbo and the ages and weight of lamb on juvenile lamb superovulation
of Xinjiang Fine Wool Sheep and to compare oocyte mature time from juvenile
lamb superovulation and adult ewe ovary and to study the effect of oocyte mature
time and oocyte level on the quality of IVF. The results showed that the optimal
method of lambs superovulation
was to inject 30 mg FSH made in Canada for four times with the interval of 12
h and the best maturation time of oocyte in vitro for cleavage was 30
h. The development of oocyte in different stages was not affected on cleavage.
This study can provide important experimental data for juvenile in vitro
embryo technology.
Keywords: Xinjiang Fine Wool lamb;follicular development;oocyte;maturation in vitro;IVF