Mingfei  Sun, Shenquan  Liao, Jianfeng  Yuan, Zonghua  Qin, Caiyan  Wu, Minna  Lv, Nanshan  Qi, Jianping  Cai, 
    Biochemical Characterization of the Outer Membrane Enzyme OMPLA from <I>Riemerella anatipestifer</I>,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 21,
    2012,
    Pages 4073-4078,
    ISSN 1680-5593,
    javaa.2012.4073.4078,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.4073.4078)
    Abstract: A gene (<I>pldA</I>) encoding a 33.0 kDa protein with significant homology to the <I>Escherichia coli</I> outer membrane phospholipase A was identified from <I>Riemerella anatipestifer</I> genome database. The results of this study revealed that this gene encoded an Outer Membrane Phospholipase A (OMPLA) in <I>Riemerella anatipestifer</I>. The recombinant OMPLA protein was expressed and its general biochemical features were also, determined. Methyl Arachidonyl Fluorophosphonate (MAFP) could specifically inhibit the OMPLA activity. These observations suggest that OMPLA could be exploited as a drug target in the bacteria and MAFP may serve as a lead compound for drug discovery against <I>R. anatipestifer</I>.
    Keywords: Riemerella anatipestifer;outer membrane phospholipase A;methyl arachidonyl fluorophosphonate;protein;database