S.H. Chen, Y.H. Chu, J.X. Chen, L. Ai, Y.C. Cai, Y.L. , J. Li, G.S. He,
Detection of Cryptosporidium Species in Feces from Snakes,
Journal of Animal and Veterinary Advances,
Volume 11,Issue 21,
2012,
Pages 4030-4033,
ISSN 1680-5593,
javaa.2012.4030.4033,
(https://makhillpublications.co/view-article.php?doi=javaa.2012.4030.4033)
Abstract: Cryptosporidiosis is an important gastrointestinal disease in snakes. In the current study, 143 feces samples of cobra snakes and Oriental rat-snake were examined for the presence of cryptosporidia by morphology and Polymerase Chain Reaction (PCR) Method targeting a part of the 18S ribosomal RNA gene. A consecutive sequencing reaction was used to identify the cryptosporidian species present in PCR-positive samples. The oocyst of Cryptosporidium serpentis was found in 19 out of 143 (13.29%). The results stress the importance for diagnostic methods to be specific for Cryptosporidium species especially in snakes.
Keywords: Cryptosporidium serpentis;snakes;morphology;polymerase chain reaction;prevalence;sequences