Jesus Genaro  Sanchez-Martinez, Roberto  Perez-Castaneda, Ma. De la Luz  Vazquez Sauceda, Jaime Rabago  Castro, Gabriel  Aguirre-Guzman, 
    Rapid Detection of <I>Edwardsiella ictaluri</I> from Channel Catfish Tissue and Water Samples by PCR Amplification,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 20,
    2012,
    Pages 3823-3826,
    ISSN 1680-5593,
    javaa.2012.3823.3826,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.3823.3826)
    Abstract: Polymerase Chain Reaction (PCR) primers were designed to detect 
  <I>Edwardsiella ictaluri</I> in tissue and water samples. Three pairs of primers 
  were designed and tested against the <I>E. ictaluri</I> type strain (ATCC No. 
  33202). The primers for pEI1 and EIl6s were very sensitive for the detection 
  of <I>E. ictaluri</I> in water and tissue samples of fish experimentally infected 
  with different bacterial dilutions. The PCR conditions described were optimized 
  to obtain results in 5 h. This PCR has the potential of detecting low levels 
  of <I>E. ictaluri</I> in tissue and water samples allowing for the timely detection 
  of enteric septcaemia of catfish in farms.
    Keywords: PCR;detection;E. ictaluri;catfish;Ell6s