Ying  Li, Youjin  Hao, Yujuan  Zhang, Yang  Gao, 
    Comparison of Lipogenesis Capacity of Grass Carp (<em>Ctenopharyngodon idella</em>) 
  Preadipocyte from Different Anatomical Sites and Body Weight,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 3,
    2012,
    Pages 375-380,
    ISSN 1680-5593,
    javaa.2012.375.380,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.375.380)
    Abstract: Fat cells in adipose tissue turn over throughout the life span. The objective 
  of this study was to explore the cellular and molecular basis for regional and 
  body weight differences in grass carp (<em>Ctenopharyngodon idell</em>a) preadipocyte. 
  The number of fresh isolated preadipocytes were counted from different body 
  weight and anatomical sites using the hemocytometer and the proliferation capacity 
  of the cells was tested by MTT assay. In addition, the differentiation capacity 
  of cells was assessed through mRNA expression of Peroxisome Proliferator-Activated 
  Receptor-&gamma; ( (PPAR-&gamma;) and cellular Triglycerides (TG) content. The 
  total number and proliferative capacity of visceral cells was significantly 
  (p&lt;0.001) higher compared with cells in the back subcutaneous and there was 
  a very significant (p&lt;0.001) decline in the proliferative capacity of cells 
  with the increase of body weight. The highest TG value and mRNA level of PPAR&gamma; 
  were obtained in the visceral cells from 5000 g fish and the TG value, mRNA 
  level of PPAR&gamma; in cells increased to a greater extent with the increase 
  of fish body weight, irrespective of the depot origin. Moreover, in the case 
  of the same weight of fish, there was a greater differentiation degree in visceral 
  cells than in back subcutaneous cells. In summary, preadipocyte lipogenic capacity 
  of growing fish depends mainly on fat depot origin and significantly greater 
  lipogenic capacity of the fish&#146;s adipose tissue is localized visceral. 
  Furthermore, the proliferation capacity of fat cell declines with the increase 
  of body weight and the increasing weight during the growth of healthy fish enhances 
  the lipogenic capacity. Researchers conclude that fat cells from different regions 
  and body weight are inherently distinct.
    Keywords: lipogenesis capacity;Anatomical sites;grass carp;body weight;preadipocyte;China