Hong-Ning  Wang, Wen-Qiao  Fan, Yi  Zhang, An-Yun  Zhang, 
    Evaluation of the Suitability of Five Housekeeping Genes as Internal Control for Quantitative Real-Time RT-PCR Assays in Chickens Acutely Infected with IBV M41 Strain,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 17,
    2012,
    Pages 3064-3067,
    ISSN 1680-5593,
    javaa.2012.3064.3067,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.3064.3067)
    Abstract: Avian Infectious Bronchitis (IB) is an acute and highly contagious 
  respiratory disease in chickens. To detect the virus in chickens, housekeeping 
  genes were detected by quantitative real-time PCR for the normalization. The 
  aim of study was to identify the most stable housekeeping gene (s) in IBV infection. 
  Five housekeeping genes including <I>18S rRNA</I>, <I>&#946;-actin</I>, Glyceraldehyde-3-Phosphate 
  Dehydrogenase (<I>GAPDH</I>), Glucose-6-Phosphate Dehydrogenase (<I>G6PDH</I>) 
  and Ubiquitin (<I>UB</I>) were investigated in nine tissues: trachea, thymus, 
  liver, spleen, lungs, kidney, pancreas, proventriculus and bursa of Fabricius. 
  The geNorm and Normfinder Software were used to calculate the stability of the 
  candidate genes.
    Keywords: Avian infectious bronchitis virus;housekeeping genes;quantitative real-time reverse transcription-polymerase chain reaction;suitability;China