Yi-Bing  Chen, Song  Gao, Jun-Fang  Zhou, Xi-He  Wan, 
    Establishment of a Multiplex PCR and an Investigation of Co-Infection Rate of WSSV and IHHNV in <I>Penaeid vannamei</I> in Northern of Jiangsu,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 2,
    2012,
    Pages 181-185,
    ISSN 1680-5593,
    javaa.2012.181.185,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.181.185)
    Abstract: Based on the conservative regions in genome of WSSV and IHHNV, two specific primer sets, respectively for the two viruses were designed and constructed. A multiplex PCR for co-detection of the two viruses with a specificity of (IHHNV, TSV, YHV,Vibrio Parahaemolyticus, etc.) and sensitivity of 1x10<SUP>3</SUP> template copy numbers was subsequent established after serially optimization. WSSV and IHHNV with different template concentrations could be detected simultaneously. By the multiplex PCR method, 265 diseased shrimp samples and 169 healthy shrimp samples collected from the farms in the Northern area of Jiangsu province was investigated for WSSV and IHHNV co-infection rate in pond-cultured <I>P. vannamei</I>. The results show that the infection rate of WSSV was quite high (45~54%) whereas that of IHHNV was very low (6.9~13.7%). The result also indicated that there were 7~10% of diseased shrimp and 0~6.7% of healthy shrimp were co-infected with the two viruses indicated that co-infection of the two viruses was more popular in diseased shrimp than the healthy.
    Keywords: templates;China;co-infection;multiplex PCR;infectious hypodermal and hematopoietic necrosis virus;White spot syndrome virus