Yongmei  Su, Wenjin  Zhu, Jianhua  Wu, 
    Polymorphism Analysis on Intron 3 of the <I>GH</I> Gene in Chinese Donkey,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 10,
    2012,
    Pages 1705-1707,
    ISSN 1680-5593,
    javaa.2012.1705.1707,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.1705.1707)
    Abstract: This experiment was conducted to study the polymorphism on intron 3 of <I>GH</I> gene in donkey in China. The polymorphism was analysised by PCR-SSCP in the 10 donkey breeds (JN, GL, GZ, DZ, JM, BY, QY, LZ, TH and XJ) by PCR-SSCP. One SNPs was found in the sequence of <I>GH</I> gene through the sequencing analysis of the two homozygous genes <I>AA</I> and <I>BB</I>, the mutation at 100th site (G&rarr;T) of the third intron of donkey <I>GH</I> gene was showed. Three genotypes AA, AB and BB were detected in 10 donkey breeds and the allele A was the predominant allele. All the breeds were in Hardy-Weinberg equilibrium at this polymorphic site (p&gt;0.05). The results confirmed that there were polymorphisms in the intron 3 of <I>GH</I> gene.
    Keywords: Donkey;GH gene;intron 3;polymorphism;PCR-SSCP;China