Nining  Hong, Qian  De-Xing, Zhang  Deng-Xiang, Ran  Long-Zhong, 
    A Simple Cultural Method for Detection of <I>Mycoplasma bovis</I>,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 10,
    2012,
    Pages 1643-1646,
    ISSN 1680-5593,
    javaa.2012.1643.1646,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.1643.1646)
    Abstract: Simple method for the detection of <I>Mycoplasma bovis</I> in both liquid and solid cultures was established. Fresh media were easily prepared and dispatched into an eppendorf tube or a Petri dish, a new cultural procedure was followed with the eppendorf tube closed or the Petri dish sealed. Compared to the conventional methods, appropriate inoculating and subculturing operations got easier and a CO<SUB>2</SUB> incubator was not required; moreover, probable contamination by airborne fungi or bacteria could be reduced or eliminated during long-term incubation. Similarly, <I>M. bovis</I> grew normally and could be investigated by Color-Change Units (CCU) enumeration, colony morphology or PCR assay.
    Keywords: Culture;detect;method;Mycoplasma bovis;media;CO2