Guoxing Nie  , Xianglin  Cao, Jianjun Chen  , Haijin Liu  , Junli Wang  , 
    Study of Enzymolysis Kinetics with Four Kinds of Protein Feeds in <I>Megalobrama amblycephala in vitro</I>,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 9,
    2012,
    Pages 1378-1384,
    ISSN 1680-5593,
    javaa.2012.1378.1384,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.1378.1384)
    Abstract: This research provides a comparative study on the ability of protein digestion and enzymolysis with 4 kinds of common feed ingredients in <I>Megalobrama amblycephala in vitro</I>. About 12 <I>Megalobrama amblycephala </I>with were temporarily fed a day for eliminating the contents of digestive canal. The intestine and hepatopancreas were took out for the preparation of the digestive (I&#146;ve juice sample) for digesting four kinds of common feed ingredients (fish meal, soybean meal, rapeseed meal and cottonseed meal) by enzymatic hydrolysis <I>in vitro</I>. The results showed that compared with the hepatopancreas, the intestine of <I>Megalobrama amblycephala</I> possessed better enzymolysis ability for protein in feed ingredients. Moreover, the enzymolysis ability of post intestine was the highest and the lowest in former intestine. The enzymolysis rate of fish meal (6.543 mg h<SUP>-1</SUP>) in intestine within 0-4 h was higher than that of other feeds also the enzymolysis rate of fish meal (1.781 mg h<SUP>-1</SUP>) was the highest in hepatopancreas than others (p&lt;0.05).
    Keywords: Digestive in vitro;feed ingredients;enzymolysis kinetics;Megalobrama amblycephala;hepatopancreas;China