Mingfei Sun  , Caiyan  Wu, Shenquan Liao  , Nanshan Qi  , Xinyu Peng,  , Minna Lv  , 
    Molecullar Typing, Prevalence of netB and Antimicrobial Susceptibility among Clinical Isolates of <I>Clostridium perfringens</I> from Chickens in Southern China,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 8,
    2012,
    Pages 1183-1187,
    ISSN 1680-5593,
    javaa.2012.1183.1187,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.1183.1187)
    Abstract: <I>Clostridium perfringens </I>is an important pathogen of animals and humans and is the causative agent of Necrotic Enteritis (NE) in poultry. This study focused on the typing of intestinal <I> C. perfringens</I> isolates (n = 78, 54 from chickens with NE, 24 from healthy chickens) from China&#146;s Southern Guangdong province. All isolates were classified as type A using multiplex PCR assay. The recently described toxin gene associated with NE in chickens, netB was found in 21 (26.9%) of the isolates (18 from chickens with NE and 3 from unrelated chickens with no evidence of NE). The <I>in vitro</I> susceptibility results showed that ceftriaxone showed highest activity with lowest MIC<SUB>50</SUB> values (0.25-0.5 &#956;g mL<SUP>-1</SUP>) followed by cefotaxime (0.25-4 &#956;g mL<SUP>-1</SUP>), penicilin (0.5-2 &#956;g mL<SUP>-1</SUP>). However, most of the isolates were resistant to lincomycin, tetracycline, metronidazole and neomycin as evident by the high MIC<SUB>50</SUB>. Furthermore, 70.5% of the isolates (n = 55) were simultaneously resistant to &gt;4 antibiotics and 30.8% of the isolates (n = 24) were resistant to all the antimicrobials tested. To the knowledge this is the first study of the prevalence of <I>C. perfringens </I>in broiler chickens in China and the first to examine the isolates for the recently identified novel toxin genes, <I>netB</I>.
    Keywords: China;Clostridium perfringens;molecullar typing;antimicrobial susceptibility;isolates;genes