Liying  Zhang, Wenjun  Yang, Zongyi  Wang, Yiqiang  Chen, Binru  Shang, 
    Development and Validation of a Gas Chromatography-Mass Spectrometry Method for the Simultaneous Determination of Melamine and Cyromazine in Animal Feeds,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 1,
    2011,
    Pages 73-80,
    ISSN 1680-5593,
    javaa.2011.73.80,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.73.80)
    Abstract: A new method for simultaneous determination of melamine and cyromazine in animal feeds using Gas Chromatography-Mass Spectrometry (GC-MS) was developed and validated. Samples were extracted with trichloroacetic acid solution cleaned up by cation exchange solid-phase extraction cartridges and derivatized with N, O-bis (trimethylsilyl) trifluroacetamide containing 1% trimethylchlorosilane followed by GC separation and MS detection. The limits of quantification were 0.10 mg kg<SUP>-1</SUP> for both melamine and cyromazine. Recoveries from feeds spiked at levels between 0.1 and 50 mg kg<SUP>-1</SUP> ranged from 84.2-99.5% with Relative Standard Deviation (RSD) &lt;8% with the exception of a 10.2% RSD for 0.1 mg kg<SUP>-1</SUP> melamine. This validated method was successfully applied to commercial feed samples showing that it can be used as a routine tool for the surveillance and evaluation of the presence of melamine and cyromazine in animal feeds.
    Keywords: determination;cyromazine;gas chromatography-mass spectrometry;feeds;Melamine