Liu  Yonggang, 
    A Novel Pig Gene, PFKFB1, Differentially Expressed in the Muscle Tissues from Wujin Pigs and Large White Pigs,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 4,
    2011,
    Pages 421-427,
    ISSN 1680-5593,
    javaa.2011.421.427,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.421.427)
    Abstract: The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large white pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence of the gene was obtained using the Rapid Amplification of Cdna Ends (RACE) method. The open reading frame of this gene encodes a protein of 428 amino acids which is homologous with the 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 1 (PFKFB1) of 6 species: cattle (97%), human (97%), horse (96%), rhesus monkey (96%), rat (94%) and mouse (94%). This newly identified gene was respectively defined as the swine PFKFB1 gene and had been assigned GeneID: 100233197. The phylogenetic tree analysis revealed that the swine PFKFB1 gene has a closer genetic relationship with the PFKFB1 gene of cattle. The tissue expression analysis indicated that the swine PFKFB1 gene has a broad tissue distribution. The experiment is the first to establish the primary foundation for further research on the swine PFKFB1 gene.
    Keywords: gene;Pig;mRNA;differential display;muscle tissue;PFKFB1;China