R.Q. Lin, Y.R. Zhao, Y.G. Ye, R.L. Zhang, X.Q. Zhu, X.H. Li, C.Y. Liu, S.T. Gao, Y.J. Geng, D.N. Huang,
Identification of One Putative D7 Related Gene Specifically Expressed in Female Anopheles anthropophagus,
Journal of Animal and Veterinary Advances,
Volume 10,Issue 20,
2011,
Pages 2618-2623,
ISSN 1680-5593,
javaa.2011.2618.2623,
(https://makhillpublications.co/view-article.php?doi=javaa.2011.2618.2623)
Abstract: Anopheles anthropophagus is an important mosquito vector transmitting malaria and Brugia malayi. Due to differences in feeding behaviors between the female and male adult An. anthropophagus, female An. anthropophagus plays a more important role in transmitting pathogens than male ones. The present study identified a gene specifically expressed in female An. anthropophagus from a subtractive cDNA library for female An. anthropophagus and its full-length cDNA was amplified by 3' and 5' Rapid Amplification of cDNA Ends (RACE) technique. This gene was named fsyz and further analyzed by using Northern blot and bio-informatics analyses. It was shown that the fsyz could encode a D7 related salivary protein and was female-specifically expressed and not characterized previously worth further studies.
Keywords: sequence analysis;salivary protein;Northern blot;RACE;RT-PCR;Anopheles anthropophagus