Qing-Hui  Jia, Chen  Juan, Shen  Ping, Zhi-Xin  Fu, Zheng-Zhu  Liu, D.U.  Li-Xin, Chuan-Sheng  Zhang, Chun-Guang  Yin, Li-Ying  Geng, Xie-Rong  Liu, 
    Derivation and Characteristics of ES-Like Cells in Chicken Fetal Ovarian,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 2,
    2011,
    Pages 241-245,
    ISSN 1680-5593,
    javaa.2011.241.245,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.241.245)
    Abstract: In the present study, the ES-like cells were derived from chicken fetal ovarian. These cells exhibit features similar to those of chicken EG cells such as typical bird nest morphology, strong reactivity toward periodic acid-Schiff and alkaline phosphatase staining. They could be maintained for more than a month on STO feeder<I> in vitro</I>, expressed the pluripotent markers such as stage specific embryonic antigen-1, stage-specific embryonic antigen-4, tumor rejection antigen-1-60 and tumor rejection antigen-1-81. Furthermore, the ES-like cells could form embryoid bodies and differentiate to several kinds of cells<I> in vitro</I>. Their easy accessibility makes these cells an attractive alternative to chicken ES cells for future functional and genomic studies and comparison with other stem cells.
    Keywords: genome;Embryonic stem cells;n vitro culture;comparison;fetal chicken ovarian;China