Song-Jia  Lai, Jie  Wang, Wei  Wang, Si-Yi  Chen, Jia  Yao, Ming  Zhang, Yu-Mei  Feng, 
    Effects of Interferon-Tau (IFN-τ) and Progesterone on Transcription Level of Matrix Metalloproteinases-2 (MMP-2) and Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) in Cultured Bovine Endometrial Cells,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 14,
    2011,
    Pages 1833-1840,
    ISSN 1680-5593,
    javaa.2011.1833.1840,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.1833.1840)
    Abstract: Interferon-tau (IFN-&#964;) secreted by blastular trophectoderm was thought to be the primary signal for pregnancy recognition during bovine early pregnancy period after pregnancy recognition, progesterone dominated pregnancy maintaining. Moreover, Matrix Metalloproteinases-2 (MMP-2) and Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) was important for embryo implantation. The objectives of studies were evaluated whether IFN-&#964; and progesterone regulated MMP-2 and TIMP-2 expression in cultured bovine Endometrial Cells (bECs). In the studies, bECs were cultured in DMEM-Ham&#146;s F12 medium with free-serum. These cultured cells were divided into 4 groups and cultured in four types culture medium: culture medium without IFN-&#964; and progesterone (Co group); culture medium including 100 ng mL<SUP>-1</SUP> IFN-&#964; (I<SUB>N</SUB> group); culture medium including 200 nmol L<SUP>-1</SUP> progesterone (Pr group); culture medium including 100 ng mL<SUP>-1</SUP> IFN-&#964; and 200 nmol L<SUP>-1</SUP> progesterone (I+P group). Cultured bECs were harvested and extracted total mRNA after being cultured for 1, 3, 6 and 12 h and then expression level of MMP-2 and TIMP-2 were assessed with fluorescence quantitative PCR. The results showed that MMP-2 expression level in Pr, I<SUB>N</SUB> and I+P group were extremely significantly higher than in Co group (p&lt;0.01). MMP-2 expression level in I<SUB>N</SUB> and I+P group was significantly higher than in Pr group (p&lt;0.05). MMP-2 expression level was no statistical difference between I<SUB>N</SUB> group and I+P group (p&gt;0.05); TIMP-2 expression level in Pr, I<SUB>N</SUB> and I+P group was extremely significantly lower than in Co group (p&lt;0.01) and TIMP-2 expression level was no statistical difference among Pr, I<SUB>N</SUB> and I+P group (p&gt;0.05). The conclusion indicated progesterone and IFN-&#964; induced MMP-2 expression <I>in vitro</I> cultured bECs whereas, progesterone and IFN-&#964; suppressed TIMP-2 expression <I>in vitro</I> cultured bECs.
    Keywords: bovine endometrium;TIMP-2;progesterone;IFN-Ä;MMP-2;China