E.P. Aba  Adulugba, W.S.  Ezema, J.O.A.  Okoye, J.N.  Luther, 
    Histopathological Changes Associated with Epizootic of African Swine Fever in Nigeria,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 14,
    2011,
    Pages 1829-1832,
    ISSN 1680-5593,
    javaa.2011.1829.1832,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.1829.1832)
    Abstract: Twelve outbreaks of African swine fever were studied in Nsukka and Enugu areas of Enugu state, Nigeria. Mortalities were 90-100% in all the farms. Spleen and lymph nodes were swollen. Section of the spleen showed infarcts. Both spleen and lymph nodes had severe necrosis and depletion of lymphocytes. Massive infiltration of the bronchioles and alveoli by eosinophilic exudates occurred in the lungs. Dilatation of the sinusoids and eosinophilic intranuclear inclusions were observed in the liver. The outbreaks were diagnosed by ASF antibody detection in serum samples by indirect enzyme linked immunosorbent assay and by antigen detection in lymph nodes by immunofluorescent test. It was concluded that the virus involved in the outbreaks are similar to the existing pathotypes.
    Keywords: Nigeria;African swine fever;enzyme liked immunosorbent assay;pandemic;histopathology;pig;immunofluorescent test