Jon  Hickford, Tulay Ifakat  Cagatay, 
    Serotyping <I>Dichelobacter nodosus</I> with PCR-SSCP,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 13,
    2011,
    Pages 1678-1682,
    ISSN 1680-5593,
    javaa.2011.1678.1682,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.1678.1682)
    Abstract: In this study, we reported rapid approach for identification of <I>D. nodosus</I> serotypes that causes footrot, higly contagious diseases of the ruminants in worldwide with using Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) analysis. A total of 500 swab samples from infected hoof lesion were investigated for presence of <I>D. nodosus</I> by PCR amplification of <I>fimA</I> gene. PCR-SSCP patterns revealed that each sample contained two or three different <I>D. nodosus</I> serotypes. Additionally on the of basis SSCP patterns, 8 different serogroups (A- G and I) were identified from New Zealand samples. These findings were confirmed by cloning and sequences of <I>fimA</I> gene.
    Keywords: Single Strand Conformational Polymorphism (SSCP);PCR;fimA gene;Dichelobacter nodosus;sequencing;sample