T.  Yoshida, R.  Takayanagi, T.  Sanada, H.  Furuta, 
    Introduction of Exogenous Gene into Chicken PGCs via Blastoderm,
    Journal of Animal and Veterinary Advances,
    Volume 9,Issue 4,
    2010,
    Pages 748-750,
    ISSN 1680-5593,
    javaa.2010.748.750,
    (https://makhillpublications.co/view-article.php?doi=javaa.2010.748.750)
    Abstract: It was proved that an exogenous gene was successfully transferred to chicken embryo and Primordial Germ Cells (PGCs) by the injection of the blastoderm. In this experiment, an exogenous gene, Lac Z constructs encoding <I>Escherichia coli</I> beta-galactosidase was introduced into chicken blastoderm (stage X) just ovoposition using lipofection reagent. After injection of gene the eggs were incubated in routine manner until developmental stage 12-15. Survival rate of the treated embryo was 35.6%. Lac Z expression was mosaic manner and low efficiency. However, it was obtained Lac Z specific band from chicken PGCs by PCR method.
    Keywords: blastoderm; PGCs;gene transfer;Chicken embryo;exogenous gene;Lac Z