Nianli  Zou, Fangfang  Zhao, Ping  Liu, Sanjie  Cao, Xintian  Wen, Yong  Huang, 
    The Expression and Characterization of Highly Antigenic Region of Spike Protein of Prevalent Infectious Bronchitis Virus in <I>Escherichia coli</I>,
    Journal of Animal and Veterinary Advances,
    Volume 9,Issue 8,
    2010,
    Pages 1267-1274,
    ISSN 1680-5593,
    javaa.2010.1267.1274,
    (https://makhillpublications.co/view-article.php?doi=javaa.2010.1267.1274)
    Abstract: The serotypes and genotypes of Infectious Bronchitis Virus (IBV) was mainly determined by the Spike (S)1 glycoprotein. A prevalent IBV strain ZY3 was isolated and the highly antigenic region of its S1 gene was amplified and expressed in <I>Escherichia coli </I>using the pET-32a (+) vector. The fusion protein, which was expressed at a high level was similar antigenically to the native S1 protein as determined by Western blot assay using rabbits polyclonal antibodies against IBV ZY3 strain. The fusion protein was also purified. This research lays the foundation for using this recombinant protein for development of indirect Enzyme-Linked Immunosorbent Assay (ELISA) for serum antibody detection or for production of monoclonal antibodies against prevalent IBV.
    Keywords: Infectious Bronchitis Virus (IBV);Isolation;S1 gene;prokaryotic expression;pET-32a (+)