S. Tabatabaei , R.A. Batavani , A.R. Talebi , Comparison of Semen Quality in Indigenous and Ross Broiler Breeder Roosters, Journal of Animal and Veterinary Advances, Volume 8,Issue 1, 2009, Pages 90-93, ISSN 1680-5593, javaa.2009.90.93, (https://makhillpublications.co/view-article.php?doi=javaa.2009.90.93) Abstract: The aim of this study, was to evaluate the effect of rooster strain (Indigenous and Ross-308 broiler breeder roosters) on semen quality. Total of 90 semen samples were collected from 15 Ross-308 and 15 Indigenous roosters at 3 days interval. Semen was collected from roosters by abdominal massage. After dilution, semen samples were examined microscopically for quality parameters (sperm concentration, live-dead proportion, motility rate and morphological defects of sperm). The concentration of spermatozoa in Indigenous and Ross broiler breeder roosters were 3.3±0.38x109 and 6.6±0.53x109 ML-1, respectively (p<0.01). The motility rate of spermatozoa in ross roosters were 78±1.7% and significantly (p<0.01) differed from those of indigenous roosters (86.5±0.78%). The viability rate in ross males were 82.25±2.04% and in indigenous roosters were 89.63±1.32% (p<0.01). The percentage of morphological defects in ross and indigenous males were 10.13±0.40 and 7.00±0.53%, respectively (p<0.01). Total acrosome, head, mid-piece and tail abnormalities in ross roosters were 12.55±0.33, 25.91±0.13, 20.55±0.07 and 41.04±0.19%, while in Indigenous roosters were 14.31±0.18, 31.4±0.24, 10.15±0.11 and 44.1±0.26%, respectively and the differences were significant (p<0.01). It is concluded that semen quality could be different between genetically improved (Ross-308 strain) and indigenous roosters. Keywords: Cock;strain;breeder;sperm quality