Wang  Zhisheng, Ai  Dawei, Zhou  Anguo, 
    Protective Effects of Nano-ZnO on the Primary Culture Mice Intestinal Epithelial Cells in <I>in vitro</I> Against Oxidative Injury,
    Journal of Animal and Veterinary Advances,
    Volume 8,Issue 10,
    2009,
    Pages 1964-1967,
    ISSN 1680-5593,
    javaa.2009.1964.1967,
    (https://makhillpublications.co/view-article.php?doi=javaa.2009.1964.1967)
    Abstract: Nano-ZnO is a new products, which may used to be feed resources. The aim of this study was to exam, whether nano-ZnO is able to protect cell integrity from potential damage by free-radical oxidative injury. A 3x3 factorial experiment was used to investigate the interaction between particle diameter (15, 50 and 100 nm) and concentration (0.4, 1.6 and 6.4 μg mL<SUP>-1</SUP>) of the primary culture mice Intestinal Epithelium Cell (IEC) in <I>in vitro</I>. The results indicated that the all the nano-ZnO groups MTT OD value were significantly higher than the perhydrol group (p<0.05) and LDH decreased, but not significant (p>0.05). The MDA content in all the nano-ZnO groups were decreased significant (p<0.01). The SOD and CAT in all nano-ZnO groups were higher than the perhydrol group (p>0.05). The results shown that nano-ZnO released the IEC oxidative injury.
    Keywords: Intestinal epithelium cell;nano-ZnO;antioxidant;antioxidant enzymes;integrity;in vitro