Ali Hashemi  , Parviz Farhoomand  , Rasul Pirmohammadi  , Sarain Razzaghzadeh  , Mehdi Nayebpor  , 
    Effect of Extender on Sperm Motility and Acrosomal Integrity of Frozen Buffalo Sperm,
    Journal of Animal and Veterinary Advances,
    Volume 6,Issue 11,
    2007,
    Pages 1340-1342,
    ISSN 1680-5593,
    javaa.2007.1340.1342,
    (https://makhillpublications.co/view-article.php?doi=javaa.2007.1340.1342)
    Abstract: The comparative efficacy of  the  4 extender ( TEYCAFG, CUEEYG, IVTEEYG and MEEYG) was studied by split sample technique using 24 ejaculate ( 6 each from 4 buffalo bulls ) after separation of seminal plasma. The percentage of motile sperm in frozen semen was significantly higher (p< 0.01) in TEYCAFG (71.56&plusmn;1.21) and CUEEYG (67.56&plusmn;1.01) than in MEEYG (39.15&plusmn;2.27) and IVTEEYG (34.41&plusmn;3.01). The difference between the former 2 and between the latter 2 extenders was not significant. The percentage of damage acrosomes was significantly higher (p 0.05) in IVTEEYG than in TEYCAFC and CUEEYG, but the difference between MEEYG and IVTEEYG, was not significant.
    Keywords: Buffalo;extender;sperm;acrosomal integrity;frozen